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sheep igg northernlightstm nl557 (R&D Systems)

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R&D Systems sheep igg northernlightstm nl557
Sheep Igg Northernlightstm Nl557, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sheep+igg/Sheep+IgG+NorthernLights%E2%84%A2+NL557-conjugated+Antibody/bio_rxiv__64898__2026__04__14__718463-57-4-11
Average 93 stars, based on 43 article reviews

sheep igg northernlightstm nl557 - by Bioz Stars, 2026-09

93/100 stars

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Article Title: A sequence invariable region in TcdB2 is required for toxin escape from Clostridioides difficile .
Article Snippet: HRP-conjugated secondary antibodies were used at a 1:50 dilution and included Sheep IgG (RnD systems), Mouse IgG (Biotechne), and Rabbit IgG (Biotechne).

Article Title: A sequence invariable region in TcdB2 is required for toxin escape from Clostridioides difficile .
Article Snippet: Sequence differences among the subtypes of Clostridioides difficile toxin TcdB (2,366 amino acids) are broadly distributed across the entire protein, with the notable exception of 76 residues at the protein’s carboxy terminus.. This sequence invariable region (SIR) is identical at the DNA and protein level among the TcdB variants, suggesting this string of amino acids has undergone selective pressure to prevent alterations.. The functional role of the SIR domain in TcdB has not been determined.

Article Title: Cell Cycle Arrest of a ‘Zippering’ Epithelial Cell Cluster Shapes the Face and is Disrupted in Craniofacial Disorders
Article Snippet: The vector constructs for the gene fragment sequences listed below were synthesized by TWIST Bioscience and cloned into the KpnI/HindIII sites of the pNL3.2 (Nluc/minP) vector (Promega, cat: N1041).

Article Title: Veverimer, a Non-Absorbed Gastrointestinal Tract HCl Binder, Decreases Renal Ammoniagenesis and Mitigates Nephrotoxic Serum Nephritis
Article Snippet: Assay plates were coated with normal sheep IgG (R&D Systems, Cat# 5-001-A; I ug/mL) i.e., capture antigen.

Incubation:

Article Title: Embryonic inhibition of colony-stimulating factor 1 receptor impacts craniofacial morphogenesis.
Article Snippet: Next, sections were incubated with sheep anti- mouse CSF1R (10 μg/mL, R&D Systems Cat# AF3818, RRID:AB_884158) antibody overnight at 4°C followed by donkey anti- sheep Alexa Fluor 647 (1:250, Thermo Fisher Scientific Cat# A21448, RRID:AB 10374882). .. Negative controls were incubated with pre- immune sheep IgG (10 μg/mL, R&D Systems Cat# 5- 001- A, RRID:AB_10141430). ..

Immunoprecipitation:

Article Title: A druggable UHRF1/DNMT1/GLI complex regulates Sonic hedgehog dependent tumor growth
Article Snippet: For immunoprecipitations from whole cell lysates, cells were directly lysed in buffer B (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM EDTA, 1% NP-40, 1X protease inhibitor cocktail (Thermo Fisher), then subjected to the same procedure. .. The following antibodies were used for immunoprecipitation: GLI1 (prepared as described before [ 39 ]), GLI2 (Cat# AF3526, R&D Systems, RRID:AB_2279108), rabbit IgG (Cat# 12370, MilliporeSigma), sheep IgG (Cat# 5-001-A, R&D Systems). .. For the size-exclusion chromatography, a Superose 6 Increase 10/300 size-exclusion chromatography column (Cytiva) was calibrated with a high molecular weight calibration kit (Cytiva) in 150 mM NaCl, 40 mM Tris·HCl pH 7.4, 1 mM EDTA, 0.5 mM DTT, 1% glycerol, 0.001% NP-40, using an AKTA Purifier FPLC (GE Healthsciences).

Purification:

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer
Article Snippet: .. The following IgGs were included: mouse IgG1-PE conjugate (5 μL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/ml for LRIG1 R&D Systems #5-001-A), and mouse IgG1-PE conjugate (10 μL for LGR5; R&D Systems #IC002P). .. It is worth noting that only the LRIG1 was incubated with a secondary antibody conjugated to allophycocyanin (APC; R&D Systems #F0127).